Monthly Archives: December 2015

  1. Microarray Platforms: Beads, Slides or Chips?

    Microarray Platforms: Beads, Slides or Chips?

    Since its advent in 1995, miniaturized microarray analysis has become a mainstay analytical technique of genomic, proteomic, pharmaceutical and clinical laboratories worldwide. The initial microarray chips, developed by Pat Brown at Stanford University, utilized oligonucleic acid probes deposited on a substrate to discover the genomic composition of cells. After this initial focus, the technology rapidly expanded to utilize other, related probes (such as DNA, RNA, peptides, carbohydrates).

    In addition to expanding the types of molecular probes, the technology also increased the types of substrates to which probes can be attached. Glass slides, plastic/silica chips, and silica or polystyrene beads can now be used as the medium for constructing a microarray.

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  2. Cell Disruption: Sonication vs. Homogenization

    Cell Disruption: Sonication vs. Homogenization

    The preparation of samples for protein and nucleic acid analysis requires two essential steps: disruption of the tissue to release individual cells and lysis of those cells to release their cellular contents. Mechanical homogenization and sonication (sometimes referred to as ultrasonic homogenization) are two mainstay techniques for these processes.

    Selecting the proper technique for your particular application deserves careful thought. Each one has its benefits and drawbacks, and laboratories often choose to use these techniques in concert with one another. Keep reading for information to help select a method that avoids damaging precious or irreplaceable tissue samples.

    Mechanical Homogenization

    Mechanical homogenization utilizes direct physical force to bring a biological sample in solution to a state of uniform distribution, such that all fractions’ molecular composition is consistent. Traditionally, mechanical disruption was achiev

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