- Non-toxic alternative to Ethidium Bromide for nucleic acid gel staining
- Volume: 1mL
- Excitation peak:490nm (blue); 290nm (UV)
- Emission peak: ~530nm; Concentration: 20,000X
Gel Transilluminators,Electrophoresis Systems
SmartGlow Pre-stain for nucleic acid gels, 1ml
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Manufacturer SKU: E4500-PS
Manufactured By: Benchmark
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Benefits and Protocol of SmartGlow PS and LD Nucleic Acid Stains
Benefits of SmartGlow E4500-PS
- 20,000x concentration
- Replaces ethidium bromide (EB) in agarose and polyacrylamide gel electrophoresis; can also be used with UV light visualization
- Light emission: green fluorescence when bound to dsDNA or ssDNA; red fluorescence
- Excitation peaks: 490nm (blue); 290nm (UV)
Protocol for SmartGlow E4500-PS
- Use with prepared 100ml of agarose or polyacrylamide solution
- Add 5µl of SmartGlow E4500-PS Pre-Stain to gel solution prior to pouring gels
- For increased sensitivity and improved detection of small quantities of nucleic acid, add SmartGlow PS to running buffer at ratio of 5µl per 100 ml
- When electrophoresis is complete, view gel using SmartBlue Blue Light Transilluminator
Benefits of SmartGlow E4500-LD
- 6x concentration
- Add directly to samples
- Direct addition to samples eliminates chance of contaminating containers and gels
- Excitation peaks: 490nm (blue); 290nm (UV)
Protocol for SmartGlow E4500-LD
- Prepare 100ml of agarose or polyacrylamide solution
- Pour gels
- Mix SmartGlow LD with DNA markers and samples at a 1 to 5 (dye to sample) dilution
- After electrophoresis, view gel using SmartBlue Blue Light Transilluminator
2808-10
Terra Part # 2808-10
$107*
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Terra Part # 2808-10
64928
$107*
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