Yields: 10 Reactions per kit
Comprehensive kit includes all of the necessary components to synthesize single stranded aRNA from total cellular RNA starting material
KIT CONTAINS:
Enzyme Mix 1: 16.5 μl in 0.5 ml tube
Enhancer solution: 50 μl in 0.5 ml tube
Reaction buffer 1: 50 μl in 0.5 ml tube
DNTP Mix: 22 μl in 0.5 ml tube
DTT: 50 μl in 0.5 ml tube
Primer 1AS: 11 μl in 0.5 ml tube
Primer 2AS: 11 μl in 0.5 ml tube
Primer 3AS: 22 μl in 0.5 ml tube
11 μl in 0.5 ml tube
Enzyme Mix 2: 11 μl in 0.5 ml tube
Reaction Buffer 2: 150 μl in 0.5 ml tube
DNA Binding Buffer: 1.1 ml in 2 ml tube
DNA Wash Buffer Concentrate: 0.5 ml in 8 ml bottle
DNA Micro Column (without cap): 10 each
Elution Tube (1.5 ml): 10 each
2 ml Wash Tube: 10 each
Nuclease Free Water: 1.5 ml in 2 ml tube
Enzyme Mix 3: 22 μl in 0.5 ml tube
NTP Mix: 79.2 μl in 0.5 ml tube
Reaction Buffer 3: 22 μl in 0.5 ml tube
DNAse 11 μl in 0.5 ml tube: 11 μl in 0.5 ml tube
Sodium Acetate Solution: 50 μl in 0.5 ml tube
Linear Acrylamide:11 μl in 0.5 ml tube
Use in downstream applications such as DNA microarrays
Amplifies RNA for microarray, splice variant microarray and downstream applications requiring high quality aRNA
Generates 5 to 250 µg of amplified antisense mRNA (aRNA) ready for labeling
Optimized for 50ng to 1µg of total cellular RNA as starting material
Full length cDNA synthesis with unique primers
Unique second strand synthesis: takes the advantage of both linear and exponential amplifications
Minimizes loss during purification by using unique columns
Increases yield with optimized reactions with unique additives

• Manufacturer SKU: ASK

• Unit of Measure: EA

• Weight, Product: 2 lb

• Weight, Shipping: 4 lb

• Package Type: Box

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aRNA Synthesis Amplification Kit

Terra Universal (714) 578-6016

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