Product Details
- CLARIOstar Plus Multi-Mode Plate Reader with two LVF monochromators and a built-in UV/Vis spectrometer
- Provides ultra-fast data sampling of 100 measurements per second
- Dedicated laser for Alpha Technology, rapid full-plate autofocus and spectral scanning in ABS, FI and LUM
- Upgradable unit integrates into automated systems
- • Enhanced Dynamic Range simplifies assay development ideal for kinetic assays
- Applications: Assay development, Real-time cell-based assays, Signaling, Protein-protein interaction, Fast kinetics, Cell/bacterial growth, Reporter gene analysis, Enzymatic reactions, Drug discovery, Metabolism, DNA/RNA quantification, Aggregation studies
- Detection Modes: Fluorescence intensity (incl. FRET), Fluorescence polarization , AlphaScreen®/AlphaLISA®/AlphaPlexTM,, Luminescence (flash and glow) - incl. BRET, Time-resolved fluorescence, TR-FRET, UV/vis absorbance spectra
- Measurement Modes: Top and bottom reading, Endpoint and kinetic, Sequential multi-excitation, Sequential multi-emission, Spectral scanning (fluorescence, luminescence, absorbance), Ratiometric measurements, Well scanning
- Microplate Formats: 6- to 1536-well plates, user-definable. LVis Plate with 16 low volume microspots (2 μL) (sold separately)
- CLARIOstar Plus Additional Features:
- Microplate Carrier: Robot compatible
- UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
- Light Sources: High energy xenon flash lamp; Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
- Optical Filters: Excitation and emission slides for 4 filters each
- Optical Path Guides: Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
- Z-Adjustment: Automatic focal height adjustment (0.1 mm resolution)
- Shaking: Linear, orbital, and double-orbital with user-definable time and speed
- Up to two integrated barcode readers
- Incubation: +3 °C above ambient up to 45 °C or 65 °C
- Conforms to SBS standards for microplates.
- Low noise Photomultiplier Tube (PMT)
- Fluorescence Excitation / Emission Spectral Scanning
- Luminescence Emission Spectral Scanning
- Detectors: Low-noise photomultiplier tube, Red-shifted photomultiplier tube, CCD spectrometer
- Software: Multi-user Reader Control and MARS Data Analysis Software included; FDA 21 CFR Part 11 compliant
- Software Selectable Bandwidths: 8 - 100 nm
- Prevents condensation build-up on lid or sealer with upper heating plate of incubation chamber operating at 0.5 °C more than the lower plate
- Wavelength Selection
- Dual Linear Variable Filter (LVF) Monochromators™
- Linear Variable Dichroic Mirror
- Separates ex & em LVF Monochromators
- Optical filters: excitation and emission slides hold up to 4 filters each
- LVF Monochromators + optical fi liters: use one for excitation and the other for emission
- UV/vis absorbance spectrometer: full spectra or 8 discrete wavelengths in < 1 sec/well
- Optical filters: Ex and em slides hold 4 filters each, LVF Monochromators + optical filters
- Spectral Range
- Filters: FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT); LUM: 240 - 740 nm
- LVF Monochromators: FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT); LUM: 320 - 740 nm
- Linear Variable Dichroic: 340 - 740 nm or 340 - 760 nm (red-shifted PMT)
- Spectrometer: ABS 220 - 1000 nm; wavelength precision: ≤ 0.5 nm
- Accuracy: < 1% at 2 OD
- Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
- Linearity: ≤ 0.8% at 2.0 OD
- Read Times
- Flying mode (1 flash): 8 s (96), 15 s (384), 28 s (1536)
- 10 flashes: 19 s (96), 57 s (384), 3 min 4 s (1536)
- Reagent Injection
- Up to 2 built-in reagent injectors
- Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
- Variable injection speed up to 420 µL / s
- Reagent back flushing
- Optional Accessories
- LVis Plate (or 2 µL samples), THERMOstar Microplate incubator and shaker
- Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
- Stacker Magazines for up to 50 plates – continuous loading feature
- Optical Filters Excitation and emission filters
- Upgrades: Contact a product specialist for details
- LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
- AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
- Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
- 96 = 96-well microplates
- 384sv = 384-well small volume microplates
- 384g = 384-well glass bottom microplates
- 1536 = 1536-well microplates
Specs
• Type: Plate Reader
• Plate Configuration: 1536 Well
• Reader Mode: Multimode
• Manufactured by: BMG LabTech
• Manufacturer SKU: CLARIOstar Plus
• Unit of Measure: EA
• Weight, Product: 70.5 lb
• Dimensions, Product: 17.7" W x 20.07" D x 15.7" H
