Summary

  • PHERAstar FSX HTS Multimode Plate Reader with UV/VIS spectrometer and improved robotic integration
  • Three integrated barcode readers streamline assays
  • Simultaneous Dual Emission (SDE) detects assays with two emission wavelengths in one single measurement
  • Three dedicated light sources, Decay Curve Monitoring, software-controlled top/bottom reading for assay flexibility
  • Multimode shaking capabilities, on-board reagent injectors, PMTs optimized for different detection modes
  • Detection modes: UV/vis absorbance spectra, Fluorescence intensity - incl. FRET, Luminescence (flash and glow) - incl. BRET, Fluorescence polarization, Time-resolved fluorescence, TR-FRET, AlphaScreen®/AlphaLISA®/AlphaPlexTM
  • Measurement modes: Top and bottom reading, Endpoint and kinetic, Sequential multi-excitation, Sequential multi-emission, Simultaneous Dual Emission, Spectral scanning (absorbance), Real-time ratiometric measurements, Well scanning
  • Microplate formats: Up to 3456-well plates, user-definable, LVis Plate with 16 low-volume microspots (2 μL)
  • Light sources:
  • High energy xenon flash lamp
  • Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
  • Dedicated laser for TRF and TR-FRET
  • Detectors: Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes CCD spectrometer
  • Wavelength selection:
  • Optic Modules: up to six application-specific modules (includes all filters, dichroics, and etc. for an application)
  • For top and bottom reading
  • UV/vis absorbance spectrometer: full spectrum or up to 8 distinct wavelengths in < 1 sec / well
  • Spectral range filters:
  • 230 - 750 nm or 230 - 900 nm for FI, FP
  • 230 - 750 nm for LUM
  • 230 - 900 nm for TRF
  • Spectral range Spectrometer: 220 - 1000 nm for ABS
  • Sensitivity:
  • FI (top): < 0.15 pM fluorescein (384sv black, 20 μL); < 0.5 pM fluorescein (1536 black, 8 μL)
  • FI (bottom): < 1.0 pM fluorescein (384g black, 50 μL)
  • FP: < 0.5 mP SD at 1 nM fluorescein (384sv black, 20 μL); < 1.5 mP SD at 1 nM fluorescein (1536 black, 8 μL)
  • TRF with laser: < 5 fM europium (384, 80 μL); < 15 fM europium (1536, 8 μL)
  • HTRF® (black and white microplates): Reader Control Kit (Eu) after 18h (384sv, 20 μL); Delta F > 1100 % (High Calibrator); Delta F > 25 % (Low Calibrator)
  • LUM: < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL); Dynamic range: 9 decades
  • AlphaScreen® with laser: < 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)*
  • ABS with spectrometer: Full spectrum captured in < 1 s / well; Selectable spectral resolution: 1, 2, 5, and 10 nm; OD range: 0 - 4 OD; Accuracy: < 1% at 2 OD; Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
  • Read times:
  • Flying mode (1 flash): 14s (384), 27s (1536)
  • 10 flashes: 38s (384), 1 min 52 s (1536)
  • 50 flashes: 1 min 29 s (384), 5 min 18 s (1536)
  • Reagent injection:
  • Up to 2 built-in reagent injectors
  • Injection at measurement position (6 to 384-well)
  • Individual injection volumes for each well (3 to 500 μL, optionally up to 2 mL)
  • Variable injection speed up to 420 μL / s
  • Up to four injection events per well
  • Reagent back flushing
  • Additional features of PHERAstar FSX HTS Plate Reader:
  • Optical filters: Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
  • Z-adjustment: Automatic focal height adjustment (0.1 mm resolution)
  • Shaking: Linear, orbital, and double-orbital with user-definable time and speed
  • Incubation: +5 °C above ambient up to 45 °C; (incubation chamber upper heating plate operates at 0.5ºC more than lower plate to prevent condensation build-up on sealer or lid)
  • Robot compatible microplate carrier
  • Software: Multi-user Reader Control and MARS data analysis software included (FDA 21 CFR Part 11 compliant)
  • High performance module based optical system with barcode reader
  • Four PMT measurement system, optimized for specific modes
  • Rapid transport system provides fastest read times of PMT based microplate readers
  • Standard Service Contract for one year including free software upgrades
  • Optional Accessories: THERMOstar, LVis Plate, Stacker
  • Upgrades: Speak or chat with a product specialist for information on detection modes, reagent injectors, etc.

Specifications

Type: Plate Reader

Application: ELISA, Protein Assays, Nucleic Acid Assays

Wavelength Range: 220 to 1000 nm

Plate Configuration: 1536 Well

Reader Mode: Multimode

Manufactured by: BMG LabTech

Manufacturer SKU: PHERAstar

Unit of Measure: EA

Helena

Weight, Product: 108.02 lb

Dimensions, Product: 17.7" W x 20.07" D x 18.05" H

Microplate Equipment

  • Overview video of PHERAstar FSX HTS Multimode Plate Reader by BMG LabTech Overview of PHERAstar FSX HTS Multimode Plate Reader by BMG LabTech
  • Video of How BMG LabTech PHERAstar FSX and its software support drug discovery in neuroscience How the PHERAstar FSX Software support drug discovery in neuroscience
  • Video of GPCR Binding and signaling studies with the PHERAstar FSX Multimode Plate Reader by BMG LabTech GPCR Binding and signaling studies with the PHERAstar
  • Video explaining built-in Reagent Injectors for PHERAstar FSX Microplate Reader by BMG LabTech Reagent Injectors for PHERAstar FSX Microplate Reader
  • Video of Studying binding kinetics on the PHERAstar FSX by BMG LabTech Studying binding kinetics on the PHERAstar FSX
  • Video of PHERAstar FSX at Zero Gravity PHERAstar FSX at Zero Gravity
  • Video presentation of PHERAstar FSX by Applications Specialist Catherine Wark Presentation of PHERAstar FSX by Catherine Wark
Overview video of PHERAstar FSX HTS Multimode Plate Reader by BMG LabTech
Video of How BMG LabTech PHERAstar FSX and its software support drug discovery in neuroscience
Video of GPCR Binding and signaling studies with the PHERAstar FSX Multimode Plate Reader by BMG LabTech
Video explaining built-in Reagent Injectors for PHERAstar FSX Microplate Reader by BMG LabTech
Video of Studying binding kinetics on the PHERAstar FSX by BMG LabTech
Video of PHERAstar FSX at Zero Gravity
Video presentation of PHERAstar FSX by Applications Specialist Catherine Wark

Features and Benefits

Optic Module Simplifies Assays

Easy-to-configure optic modules detected by the instrument via a barcode contain all the filters needed to run a specific assay in one click

Simultaneous Dual Emission

Enables measurement of two emitted wavelengths at the same time reducing read times and increasing throughput ideal for multiplex Alpha assays

Decay Curve Monitoring (DCM)

DCM optimizes and fine-tunes assays using a TR-FRET dedicated photon counting detection system that improves signal detection and reduces background noise

LVis Low-Volume Microplate

BMG LabTech UV/Vis Absorbance spectrometer-based microplate readers are compatible with LVis Low-volume Microplate with cuvette slots for 2ul Samples (Sold Separately)




Popular Accessories
For a full list of accessories, select a product then click Choose Accessories on that product page.
  • High-performance THERMOstar Microplate Incubator by BMG LabTech

    Compatible with BMG LabTech Microplate Readers: SPECTROstar, LUMIstar, FLUOstar, CLARIOstar, PHERAstar, POLARstar and NEPHELOstar
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