• Manufacturer SKU: ASK
• Unit of Measure: EA
Increases yield with optimized reactions with unique additives | 3033-58 displayed
Microarray Products
aRNA Synthesis Amplification Kit
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Manufacturer SKU: ASK
Manufactured By: Arrayit
Summary
- Yields: 10 Reactions per kit
- Comprehensive kit includes all of the necessary components to synthesize single stranded aRNA from total cellular RNA starting material
- KIT CONTAINS:
- Enzyme Mix 1: 16.5 μl in 0.5 ml tube
- Enhancer solution: 50 μl in 0.5 ml tube
- Reaction buffer 1: 50 μl in 0.5 ml tube
- DNTP Mix: 22 μl in 0.5 ml tube
- DTT: 50 μl in 0.5 ml tube
- Primer 1AS: 11 μl in 0.5 ml tube
- Primer 2AS: 11 μl in 0.5 ml tube
- Primer 3AS: 22 μl in 0.5 ml tube
- 11 μl in 0.5 ml tube
- Enzyme Mix 2: 11 μl in 0.5 ml tube
- Reaction Buffer 2: 150 μl in 0.5 ml tube
- DNA Binding Buffer: 1.1 ml in 2 ml tube
- DNA Wash Buffer Concentrate: 0.5 ml in 8 ml bottle
- DNA Micro Column (without cap): 10 each
- Elution Tube (1.5 ml): 10 each
- 2 ml Wash Tube: 10 each
- Nuclease Free Water: 1.5 ml in 2 ml tube
- Enzyme Mix 3: 22 μl in 0.5 ml tube
- NTP Mix: 79.2 μl in 0.5 ml tube
- Reaction Buffer 3: 22 μl in 0.5 ml tube
- DNAse 11 μl in 0.5 ml tube: 11 μl in 0.5 ml tube
- Sodium Acetate Solution: 50 μl in 0.5 ml tube
- Linear Acrylamide:11 μl in 0.5 ml tube
- Use in downstream applications such as DNA microarrays
- Amplifies RNA for microarray, splice variant microarray and downstream applications requiring high quality aRNA
- Generates 5 to 250 µg of amplified antisense mRNA (aRNA) ready for labeling
- Optimized for 50ng to 1µg of total cellular RNA as starting material
- Full length cDNA synthesis with unique primers
- Unique second strand synthesis: takes the advantage of both linear and exponential amplifications
- Minimizes loss during purification by using unique columns
- Increases yield with optimized reactions with unique additives
Specifications
• Manufacturer SKU: ASK
• Unit of Measure: EA
• Weight, Product: 2 lb
• Weight, Shipping: 4 lb
• Package Type: Box