Four independent hybridization chambers. Each chamber accepts one substrate slide for a total of 4 substrates per unit
Chambers can be launched simultaneously or set to run different protocols via the user-friendly graphical user interface
Up to 60 μl of sample can be introduced into each hybridization chamber through dedicated access port. Gaskets (black), on upper portion of the lid, are used to seal chambers
Close ports prior to hybridization to prevent evaporation. System automatically delivers solutions into the 50 μl hybridization chambers when unit is programmed and substrates are in place
Chaotic advection mixing assures that all biomolecules in solution reaches every spot on the microarray via microfluidic pumps combined with a mixing loop. The extremely complex direction and speed of fluids created by the mixing loop creates a chaotic movement, a very efficient way to mix low volume liquids in the shortest period of time. The chaotic advection mixing method in the TrayMix S4 accelerates hybridization kinetics by up to 90% and increases sample yield.
Probe concentration (0.1 µM) | Solution volume(µl) | Probe quantity (pmoles) | Method | Hybridization solution | Fluorescent Mean (a.u.) | CV |
---|---|---|---|---|---|---|
Static Hybridization | 50 | 5 | cover slip | homogenous | 5500 | 0.33 |
500 | 50 | TrayMix™ S4 | homogenous | 38500 | 0.56 | |
Dynamic Hybridization | 500 | 50 | TrayMix™ S4 | homogenous | 11474 | 0.18 |
500 | 50 | TrayMix™ S4 | non homogeneous | 11823 | 0.17 | |
Average fluorescence values and coefficients of variation (CVs) measured under different hybridization conditions (2 hours of hybridization). |
Probe quantity (5 pmole) | Solution volume (µL) | Concentration (µM) | Method | Hybridization solution | Fluorescence mean (a.u.) | CV |
---|---|---|---|---|---|---|
Static hybridization | 50 | 0.1 | cover slip | Homogenous | 5500 | 0.33 |
Dynamic hybridization | 500 | 0.01 | TrayMix™ S4 | Non-homogeneous | 6452 | 0.16 |
Average value for fluorescence and CV obtained with the same quantity of targets, both with and without agitation (2 hours of hybridization). |