The various detection modes of the EnSight™ Multimode Plate Reader combines target-based and phenotypic assays that empower researchers to better understand and characterize the various pathways of cell signaling mechanisms. The EnSight is used in a broad range of applications by scientists looking to ensure data relevance and certainty in their results.
Multiple modes for increased data insight and confidence in results
Benchtop unit with high-speed cell imaging reads microplate based assays in multiple modalities in a single run
Combines labeled, label-free and well-imaging technologies
Runs multimode plate reading within the same well
Modular design allows addition of detection modes to satisfy specific applications
The EnSight™ opens up avenues of investigation and provides more insight on assays in a single run. Researchers can characterize several components of a single cell-signaling cascade in a single well with the EnSight's multiple data acquisition modalities. The fact that these data are acquired and normalized on a per cell basis allows researchers to interrogate the dependence and magnitude of amplification that occurs as the signal travels downstream.
Fluorescence, Brightfield and digital phase-contrast mode
The cell-image mode of the EnSight performs at high speed with an advanced sCMOS camera for low signal-to-background noise, laser-based autofocus and solid-state LEDs for short illumination. This modality also allows you to select fluorescence, Brightfield or digital phase-content imaging modes. All these features culminate in a well-imaging module that delivers greater physiological relevance to your analysis.
Well imaging can be used to normalize data based on an individual well's population. Traditional in vivo cell based assays required researchers to use a separate assay to quantify cell number for data normalization. The well imaging technology of the EnSight automatically makes a cell number measurement and normalizes data. This powerful feature provides laboratories with a significant return on investment by reducing the number of runs, the requirement for a separate equipment or outsourcing to core facility.
Runs multiplex assays sequentially in a 384-well plate:
Single color image: 4 minutes
Two-color image: 5 minutes
Three-color image: 6.5 minutes (approximately)
Fluorescence: enables visualization of multiple parameters per assay using up to four colors with excitation of three colors in parallel
Brightfield: provides quick image of cells without labeling, diminishes potential labeling artifacts, cell damage, assay costs, and time
Digital Phase-Contrast: images live cells that have not been labeled fluorescently, at greater resolution than Brightfield
Benefit: Quickly and easily generates per-cell data on fixed or live cells for physiologically relevant data
Applications: Cell counting/normalization, Cytotoxicity (cell toxicity, with reporter assay combined in same well), Cell migration and signaling, Phenotypic assays, Clone selection
The Corning® Epic® technology for labeled and label-free imaging allows cell-based and biochemical assays to run on one platform that delivers rich, unbiased data without interference. Label-free measurement allows multiple measurements on the same plate without affecting cell viability or need for reagents. A cell population was first measured with the label-free modality and luminescence units (LU) were quantified. The same cell population was then measured via the ATPLITE® luminescence based cell viability assay. Image A clearly demonstrates that the label-free measurement modality has no effect on cell viability.
Benefit: Characterizes cellular signaling mechanisms completely and with more sensitivity; use of labeled and label-free technologies provide more physiologically relevant data from target identification and screening to hit confirmation.
Cell-based assays: Cell toxicity, Neuron biology, Phenotypic assays, Primary and stem cells, Receptor biology, Response to any stimulus, Suspension cell culture
Biochemical assays: Aggregation, Protein- protein interactions, Small molecule binding
Ideal for target-based, biochemical and cell-based assays the powerful Alpha Technology measurement modality can be used to examine virtually any line of investigation that would traditionally be measured via an ELISA assay.
Provided AlphaLISA® and AlphaScreen® detection produces results in half the time of standard ELISA
Applications: High-throughput analysis of bio-therapeutics, Epigenetics, Protein-protein interactions, G-Protein Coupled Recepted Pathways, Kinase cascades, and many other Intracellular interactions and signaling pathways
No wash assay format provides high sensitivity and superior dynamic range
This detection mode provides robust signal strength from fewer samples by using a photomultiplier tube to boost signal strength to a measurable level. This mode reduces the cost of assays by minimizing the amount of costly reagents used in each plate. Ideal for labs working with difficult-to-transfect cells, this modality can be used to measure any assay that leverages changes in luminescence as the assay readout.
Benefits of robust signal from fewer cells:
Cells rapidly undergo extreme alterations of their DNA upon reach of confluence and
Using fewer samples more accurately reflects the normal endogenous protein and genetic make-up of the cell population
Produces accurate and measurable results from what would otherwise be an unusable signal
Applications: Cellular assays, Reporter gene, Cell proliferation, Cell toxicity and viability, Flash luminescence, Primary and stem cells
Fluorescence-based Resonance Energy Transfer (FRET) Mode
Fluorescence-based resonance energy transfer (FRET) is a powerful tool used by researchers to interrogate the interaction of two molecules. This process links one-half of a fluorophore to each molecule of interest. If the association of the molecules is sufficiently strong, the fluorophore will emit light at a particular wavelength when excited. This modality is extremely useful to labs studying signaling pathways, epigenetics, receptor-ligand binding, and most biochemical processes.
Benefit: Eliminates high background of other methods during detection of in vivo and in situ interactions
Applications: GPCRs, Protein: protein interactions, Enzyme assays, Receptor-ligand binding, Kinases, Endogenous receptors, Cytotoxicity and Cell proliferation, Methylation or Acetylation of peptide, Histone proteins
Time-Resolved Fluorescence and Time-Resolved FRET Mode
Highly sensitive time-resolved fluorescence measurements are used in biochemical and cell-based assays using premium and low concentration samples. Using TRF mode allows researchers to measure low signal intensity over time to obtain a robust signal that traditional fluorescence would fail to provide. This modality can be used to investigate a host of molecule interactions, gene expression, and other assays that would normally be measured via traditional fluorescence techniques.
High sensitivity measurement mode ideal for low concentration samples
Applications: GPCRs, Protein: protein interactions, Enzyme assays, Receptor-ligand binding, Kinases, Endogenous receptors, Cytotoxicity and cell proliferation, Methylation or acetylation of peptide, Histone proteins
Equipped with a quad-monochromator component, EnSight allows you to scan on any wavelength for the best signal-to-background ratio (on fluorescence applications).
Applications: Cell counting, Colorimetric assays, GFP, ELISA, Quantification of protein and DNA/RNA, FRET, Gene expression
Kaleido Software for EnSight Multimode Plate Reader
The included Kaleido Data Acquisition and Analysis software has an intuitive interface that quickly guides users from set up to running of multi-technology protocols. Kaleido has pre-programmed protocols for most often used applications that can be adjusted and customized according to assay requirements. Researchers can easily export data and metadata as a single file. Data can also be exported to TIBCO Spotfire.
Application Measurement Modalities for EnSight™ Multimode Plate Reader
The EnSight system is used in a broad range of lab applications. Researchers can combine multiple measurement modes in one workflow to gain more relevant data and product more reliable results. Below is an overview of relevant applications within each modality.
Well-Imaging: Cell counting/normalization, Cytotoxicity (cell toxicity) (with reporter assay combined in same well), Cell migration and signaling, Phenotypic assays, Clone selection
Cell-based assays-Response to any stimulus, Phenotypic assays, Receptor biology, Cell toxicity, Primary and stem cells, Neuron biology, Suspension cell culture
Biochemical assays-Protein- protein interactions, Small molecule binding, Aggregation
Alpha Technology: Biologics, Biomarkers, Epigenetics, Kinases, Protein: protein interactions, GPCRs
Ultra-Sensitive Luminescence: Cellular assays, Reporter gene, Cell proliferation, Cell toxicity and viability, Flash luminescence, Primary and stem cells
TRF and TR-FRET: GPCRs, Protein: protein interactions, Enzyme assays, Receptor-ligand binding, Kinases, Endogenous receptors, Cytotoxicity and cell proliferation, Methylation or acetylation of peptide, Histone proteins
Fluorescence and Absorbance: GFP, ELISA, Quantification of protein and DNA/RNA, FRET, Cell counting, Gene expression, Colorimetric assays